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Polymerase Chain Reaction (PCR)
Primers and Probes
Oligo(dT)15 Primer
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Oligo(dT)15 Primer
Oligo(dT)15 Primer
Oligo(dT)15 Primer from Promega
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Citations:
(118)
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Oligo(dT)15 Primer
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Description
Use for first-strand cDNA synthesis with a reverse transcriptase
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Oligo(dT)15 Primer
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
C1101
Target
Poly A
Quantity
20microg
Conjugate/Tag/Label
Unconjugated
Primer Type
Oligo dT Primers
Sequence
Oligo(dT)-15
Add to Compare List
Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (118)
(61)
Patient-derived oral mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemia
PloS one
January 1, 2020
Driehuis E, Oosterom N, Heil SG, Muller IB, Lin M, Kolders S, Jansen G, de Jonge R, Pieters R, Clevers H,
et al
.
cDNA synthesis
, 10.5 μl RNA was mixed with 1 μl 50 μg/ml 110 diluted
Oligo(dT) 15 Primer
(
Promega
, cat.no.
C1101
) and incubated for 5 minutes at 70˚C. After that, 8.5
GoScript Reverse Transcriptase
mastermix (
Promega
,
(62)
Effects of Dietary Vegetable Oils on Mammary Lipid-Related Genes in Holstein Dairy Cows
Animals (Basel)
December 27, 2019
Einar Vargas-Bello-Pérez, Carolina Geldsetzer-Mendoza, Nathaly Cancino-Padilla, María Sol Morales, Heidi Leskinen, Philip C. Garnsworthy, Juan J. Loor, Jaime Romero
(
Promega
®, Madison, WI, USA). Total RNA was combined with 0.5 µg/reaction
oligo (dT)15 primer
(Cat. No.
C1101
;
Promega
®, Madison, WI, USA) to a final volume of 5 µL and was incubated at 70 °C for 5 min. Next, 15
(63)
Mediator Condensates Localize Signaling Factors to Key Cell Identity Genes
Molecular Cell
December 1, 2019
Alicia V. Zamudio et al.
generated using
SuperScript II Reverse Transcriptase
(
Invitrogen
,
18080093
) with oligo-dT primers (
Promega
,
C1101
) according to manufacturer’s instructions.
Quantitative real-time PCR
was performed on Applied Biosystems
(64)
Polycystin-1 Regulates Actomyosin Contraction and the Cellular Response to Extracellular Stiffness
Scientific reports
November 12, 2019
Nigro EA, Distefano G, Chiaravalli M, Matafora V, Castelli M, Pesenti Gritti A, Bachi A, Boletta A.
#25-0500-72). cDNA was obtained by reverse transcription of extracted RNA using Oligo(dt)15 primers (
Promega
, #
C1101
) and
ImProm-II Reverse Transcriptase
(
Promega
, #
A3802
).
Quantitative Real Time PCR
analysis was performed
(65)
Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic Therapy
Journal of clinical medicine
November 5, 2019
Driehuis E, Spelier S, Beltrán Hernández I, de Bree R, M Willems S, Clevers H, Oliveira S.
cDNA synthesis
, RNA was incubated with 50 µg/mL
Oligo(dT) 15 Primer
(
Promega
, Madison, WI, USA, cat. no.
C1101
) in water for 5 min at 70 °C. Subsequently
GoScript Reverse Transcriptase
(
Promega
, Madison, WI, USA,
(66)
N-Palmitoylethanolamide-Oxazoline Protects against Middle Cerebral Artery Occlusion Injury in Diabetic Rats by Regulating the SIRT1 Pathway
International journal of molecular sciences
September 29, 2019
Fusco R, Scuto M, Cordaro M, D'Amico R, Gugliandolo E, Siracusa R, Peritore AF, Crupi R, Impellizzeri D, Cuzzocrea S,
et al
.
(67)
Long-Term Effects of Dietary Olive Oil and Hydrogenated Vegetable Oil on Expression of Lipogenic Genes in Subcutaneous Adipose Tissue of Dairy Cows
Veterinary Sciences
September 15, 2019
Einar Vargas-Bello-Pérez, Massimo Bionaz, Pietro Sciarresi-Arechabala, Nathaly Cancino-Padilla, María Sol Morales, Jaime Romero, Heidi Leskinen, Philip C. Garnsworthy, Juan J. Loor
ImProm-II® Reverse Transcription System
(
Promega
® , Madison, WI, USA). Total RNA was mixed with 0.5 µg per reaction
oligo (dT)15 primer
(Cat. No.
C1101
;
Promega
® , Madison, WI, USA) to give a final volume of 5 µL, which was incubated for 5 min at 70 ◦
(68)
miR-17-92 and miR-106b-25 clusters regulate beta cell mitotic checkpoint and insulin secretion in mice
Diabetologia
September 1, 2019
Amitai D. Mandelbaum, Sharon Kredo-Russo, Danielle Aronowitz, Nadav Myers, Eran Yanowski, Agnes Klochendler, Avital Swisa, Yuval Dor, Eran Hornstein
out by the
miRNeasy Mini Kit
(
Qiagen
, Germany). mRNA cDNA was synthesised using an oligo d(T) primer (
C1101
;
Promega
, Madison, WI, USA) and
SuperScript II reverse transcriptase
(18064-014;
Invitrogen
, Carlsbad,
(69)
The interplay between zinc and iron homeostasis in
Aspergillus fumigatus
under zinc‐replete conditions relies on the iron‐mediated regulation of alternative transcription units of
zafA
and the basal amount of the ZafA zinc‐responsiveness transcription factor
Environmental Microbiology
August 1, 2019
Rocío Vicentefranqueira, Fernando Leal, Laura Marín, Clara Inés Sánchez, José Antonio Calera
the
SuperScript III Reverse Transcriptase
(
Invitrogen
, cat. No. 18064-093) and the
oligo (dT)15 primer
(
C1101
,
Promega
). Next,
PCR
was performed using the oligonucleotide pair JA429/A3CE-T followed by a
nested PCR
(70)
ISM1 regulates NODAL signaling and asymmetric organ morphogenesis during development
The Journal of cell biology
July 1, 2019
Osório L, Wu X, Wang L, Jiang Z, Neideck C, Sheng G, Zhou Z.
of first-strand cDNA. Briefly, 2 µg of total RNA was reverse transcribed using
Oligo(dT)15 primer
(
C1101
;
Promega
) and
Moloney murine leukemia virus
reverse transcription (M170; Promega).
Real-time PCR
was
(71)
Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice
Molecular Medicine Reports
June 27, 2019
Nancy Machorro‑Rojas, Teresita Sainz‑Espu�es, Marycarmen God�nez‑Victoria, Jorge Ismael Casta�eda‑S�nchez, Rafael Campos‑Rodr�guez, Judith Pacheco‑Yepez, Maria Elisa Drago‑Serrano
Inc.). Total RNA (0.2 µg) was used as template. The RNA was mixed with 1 µl
Oligo dT 15 primer
(cat. no.
C1101
;
Promega Corporation
) and sterile
injectable
water in a total volume of 25 µl. The reaction mixture
(72)
SWI / SNF chromatin remodeling controls Notch‐responsive enhancer accessibility
EMBO reports
May 1, 2019
Zoe Pillidge, Sarah J Bray
experiments. reverse-transcribing with
M-MLV Reverse Transcriptase
(
Promega
M1705
) using Oligo(dT)15 Primers (
Promega
C1101
). cDNA was diluted fivefold before analysis with
qPCR
. The same protocol was used to extract RNA from
(73)
Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction
Molecular medicine reports
April 1, 2019
Zhao J, Meng M, Zhang J, Li L, Zhu X, Zhang L, Wang C, Gao M.
Reverse Transcriptase with buffer (cat. no. PR6502; BioTeke Corporation), oligo (dT)15 primers (cat. no.
C1101
‑20;
Promega Corporation
, Madison, WI, USA), dNTP (cat. no. PR3001, BioTeke Corporation) at 25˚C for
(74)
Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells
Cell reports
March 26, 2019
Fleskens V, Minutti CM, Wu X, Wei P, Pals CEGM, McCrae J, Hemmers S, Groenewold V, Vos HJ, Rudensky A,
et al
.
Dynabeads,
ThermoFisher
,
11452D
TGF-β,
R&D systems
,
240-B-010
RNasin
inhibitor
,
Promega
,
N2111
dNTPs,Promega,28-4065-60 Oligo dT15,
Promega
,
C1101
PEI,Polysciences,23966-1 PKB
inhibitor
VII,Calbiochem,124018 5Z-7-oxozeanol,Tocris Biosceince,
3604/1
BIO,
Tocris
(75)
Mi-2/NuRD complex protects stem cell progeny from mitogenic Notch signaling
eLife
January 29, 2019
Zacharioudaki E, Falo Sanjuan J, Bray S.
equivalent of 2 μg of RNA was reverse transcribed with random primers [Oligo(dT)15 Primers (
Promega
C1101
)] using
M-MLV reverse transcriptase
(
Promega
M531A). The cDNA products were subsequently diluted
1:5
(76)
Fasting Upregulates npy, agrp, and ghsr Without Increasing Ghrelin Levels in Zebrafish (Danio rerio) Larvae
Frontiers in Physiology
January 24, 2019
Rafael Opazo, Francisca Plaza-Parrochia, Gustavo R. Cardoso dos Santos, Gabriel R. A. Carneiro, Vinicius F. Sardela, Jaime Romero, Luis Valladares
9 | Article 1901 Opazo et al. Immaturity of Ghrelin System in
Zebrafish
Larvae primer (cat. #
C1101
,
Promega
) for a final volume of 5 µl and then incubated at 70◦ C for 5 min. Next, 15 µl of the transcription
(77)
Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival
Scientific Reports
December 1, 2018
Fengjuan Wang, Srinivasa Reddy Bonam, Nicolas Schall, Lauriane Kuhn, Philippe Hammann, Olivier Chaloin, Jean-Baptiste Madinier, Jean-Paul Briand, Nicolas Page, Sylviane Muller
synthetized from 200 ng of RNA using
ImProm-II Reverse Transcriptase
and oligo(dT)15 (
Promega
,
A3802
and
C1101
). Ten ng of cDNA were then amplified using Taqman
Real-Time PCR
Master Mixes (ThermoFisher Scientific,
(78)
The Exon Junction Complex Undergoes a Compositional Switch that Alters mRNP Structure and Nonsense-Mediated mRNA Decay Activity
Cell reports
November 27, 2018
Mabin JW, Woodward LA, Patton RD, Yi Z, Jia M, Wysocki VH, Bundschuh R, Singh G.
transfection reagent
,
Thermo Fisher
,
13778030
JetPrime transfection reagent
,
PolyPlus
,
114-01
oligo-(dT)15 Primer
,
Promega
,
C1101
Superscript III,
Invitrogen
,
18080044
Ribonuclease H
,
Promega
,
M4281
2X SYBR Green Master Mix,
Applied Biosystems
(79)
Upregulation of Ets1 expression by NFATc2 and NFKB1/RELA promotes breast cancer cell invasiveness
Oncogenesis
November 23, 2018
Kim GC, Kwon HK, Lee CG, Verma R, Rudra D, Kim T, Kang K, Nam JH, Kim Y, Im SH.
standard protocols. Reverse transcription of 1.0ug of RNA was performed using oligo (dT) primer (
Promega
:
C1101
) with
Improm II Reverse Transcription system
(
Promega
, Madison, WI) according to the manufacturer’s
(80)
c‑Jun N‑terminal kinase inhibition attenuates early brain injury induced neuronal apoptosis via decreasing p53 phosphorylation and mitochondrial apoptotic pathway activation in subarachnoid hemorrhage rats
Molecular Medicine Reports
November 9, 2018
Geng‑Qiang Ling, Xian‑Feng Li, Xu‑Hui Lei, Zhen‑Yu Wang, Dong‑Ying Ma, Yue‑Na Wang, Wei Ye
28025021
;
Invitrogen
; Thermo Fisher Scientific, Inc.). Briefly, 1 µl
oligo (dT)15 primer
(cat. no.
C1101
-20;
Promega Corporation
, Madison, WI, USA), 1 µl random primers (cat. no.
C1181
;
Promega Corporation
),
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Oligo(dT)15 Primer from Promega
Supplier Page
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Product Specs
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